Application of tissue-scale tension to avian epithelia <i>in vivo</i> to study multiscale mechanics and inter-germ layer coupling.

ABSTRACT: As cross-disciplinary approaches drawing from physics and mechanics have increasingly influenced our understanding of morphogenesis, the tools available to measure and perturb physical aspects of embryonic development have expanded as well. However, it remains a challenge to measure mechanical properties and apply exogenous tissue-scale forces in vivo, particularly for epithelia. Exploiting the size and accessibility of the developing chick embryo, here we describe a simple technique to quantitatively apply exogenous forces on the order of ~1-100 μN to the endodermal epithelium. To demonstrate the utility of this approach, we performed a series of proof-of-concept experiments that reveal fundamental and unexpected mechanical behaviors in the early chick embryo, including mech

SUBMITTER: Oikonomou P 

PROVIDER: S-EPMC11014599 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature