A specific and portable gene expression program underlies antigen archiving by lymphatic endothelial cells.

ABSTRACT: Antigens from protein subunit vaccination traffic from the tissue to the draining lymph node, either passively via the lymph or carried by dendritic cells at the local injection site. Lymph node (LN) lymphatic endothelial cells (LEC) actively acquire and archive foreign antigens, and archived antigen can be released during subsequent inflammatory stimulus to improve immune responses. Here, we answer questions about how LECs achieve durable antigen archiving and whether there are transcriptional signatures associated with LECs containing high levels of antigen. We used single cell sequencing in dissociated LN tissue to quantify antigen levels in LEC and dendritic cell populations at multiple timepoints after immunization, and used machine learning to define a unique transcriptional program

SUBMITTER: Sheridan RM 

PROVIDER: S-EPMC11014631 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature