IL-24 improves efficacy of CAR-T cell therapy by targeting stemness of tumor cells.

ABSTRACT:

Background

Cancer stem cells (CSCs) induce therapeutic resistance and may be an important barrier to cancer immunotherapy. Chimeric antigen receptor T (CAR-T) cell therapy has demonstrated remarkable efficacy in clinical settings. However, CAR-T cell therapy fails in a large proportion of patients, especially in those with solid tumors. It is unclear how CSCs mediate resistance to CAR-T cells, and whether CAR-T cells can more effectively eradicate CSCs.

Methods

In this study, the effect of CSCs on CAR-T cell therapy was determined using in vitro and in vivo assays. Subsequently, Interleukin-24 (IL-24) was expressed along with CAR in T cells. Further in vitro and in vivo tests were performed to determine the effects of IL-24 on CSCs and CAR-T cell therapy.

Results

IL-2

SUBMITTER: Zhang K 

PROVIDER: S-EPMC11015030 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature