Respiratory complex I regulates dendritic cell maturation in explant model of human tumor immune microenvironment.

ABSTRACT:

Background

Combining cytotoxic chemotherapy or novel anticancer drugs with T-cell modulators holds great promise in treating advanced cancers. However, the response varies depending on the tumor immune microenvironment (TIME). Therefore, there is a clear need for pharmacologically tractable models of the TIME to dissect its influence on mono- and combination treatment response at the individual level.

Methods

Here we establish a patient-derived explant culture (PDEC) model of breast cancer, which retains the immune contexture of the primary tumor, recapitulating cytokine profiles and CD8+T cell cytotoxic activity.

Results

We explored the immunomodulatory action of a synthetic lethal BCL2 inhibitor venetoclax+metformin drug combination ex vivo, discovering metformin ca

SUBMITTER: Turpin R 

PROVIDER: S-EPMC11015234 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature