A network of transcription factors in complex with a regulating cell cycle cyclin orchestrates fungal oxidative stress responses.

ABSTRACT:

Background

Response to oxidative stress is universal in almost all organisms and the mitochondrial membrane protein, BbOhmm, negatively affects oxidative stress responses and virulence in the insect fungal pathogen, Beauveria bassiana. Nothing further, however, is known concerning how BbOhmm and this phenomenon is regulated.

Results

Three oxidative stress response regulating Zn₂Cys₆ transcription factors (BbOsrR1, 2, and 3) were identified and verified via chromatin immunoprecipitation (ChIP)-qPCR analysis as binding to the BbOhmm promoter region, with BbOsrR2 showing the strongest binding. Targeted gene knockout of BbOsrR1 or BbOsrR3 led to decreased BbOhmm expression and consequently increased tolerances to free radical generating compounds (H_2</

SUBMITTER: Kan Y 

PROVIDER: S-EPMC11015564 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature