Detection of P. malariae using a new rapid isothermal amplification lateral flow assay.

ABSTRACT:

Background

While Plasmodium falciparum and Plasmodium vivax cause the majority of malaria cases and deaths, infection by Plasmodium malariae and other Plasmodium species also causes morbidity and mortality. Current understanding of these infections is limited in part by existing point-of-care diagnostics that fail to differentiate them and have poor sensitivity for low-density infections. Accurate diagnosis currently requires molecular assays performed in well-resourced laboratories. This report describes the development of a P. malariae diagnostic assay that uses rapid, isothermal recombinase polymerase amplification (RPA) and lateral-flow-strip detection.

Methods

Multiple combinations of custom RPA primers and probes were designed using publicly available P. malariae genom

SUBMITTER: Assefa A 

PROVIDER: S-EPMC11015614 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature