ALPL regulates pro-angiogenic capacity of mesenchymal stem cells through ATP-P2X7 axis controlled exosomes secretion.

ABSTRACT:

Background

Early-onset bone dysplasia is a common manifestation of hypophosphatasia (HPP), an autosomal inherited disease caused by ALPL mutation. ALPL ablation induces prototypical premature bone ageing characteristics, resulting in impaired osteogenic differentiation capacity of human bone marrow mesenchymal stem cells (hBMMSCs). As angiogenesis is tightly coupled with osteogenesis, it also plays a necessary role in sustaining bone homeostasis. We have previously observed a decrease in expression of angiogenesis marker gene CD31 in the metaphysis of long bone in Alpl^+/- mice. However, the role of ALPL in regulation of angiogenesis in bone has remained largely unknown.

Methods

Exosomes derived from Normal and HPP hBMMSCs were isolated and identified by ultracentr

SUBMITTER: Dong J 

PROVIDER: S-EPMC11015668 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature