Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly.

ABSTRACT:

Background

Microbial cell surface display technology allows immobilizing proteins on the cell surface by fusing them to anchoring motifs, thereby endowing the cells with diverse functionalities. However, the assessment of successful protein display and the quantification of displayed proteins remain challenging. The green fluorescent protein (GFP) can be split into two non-fluorescent fragments, while they spontaneously assemble and emit fluorescence when brought together through complementation. Based on split-GFP assembly, we aim to: (1) confirm the success display of passenger proteins, (2) quantify the number of passenger proteins displayed on individual cells.

Results

In this study, we propose two innovative methods based on split-green fluorescent protein (split-GFP),

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11015686 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature