Efficient derivation of embryonic stem cells and primordial germ cell-like cells in cattle.

ABSTRACT: The induction of the germ cell lineage from pluripotent stem cells (in vitro gametogenesis) will help understand the mechanisms underlying germ cell differentiation and provide an alternative source of gametes for reproduction. This technology is especially important for cattle, which are among the most important livestock species for milk and meat production. Here, we developed a new method for robust induction of primordial germ cell-like cells (PGCLCs) from newly established bovine embryonic stem (bES) cells. First, we refined the pluripotent culture conditions for pre-implantation embryos and ES cells. Inhibition of RHO increased the number of epiblast cells in the pre-implantation embryos and dramatically improved the efficiency of ES cell establishment. We then determined suitable cu

SUBMITTER: Shirasawa A 

PROVIDER: S-EPMC11017101 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature