Interferon-α stimulates DExH-box helicase 58 to prevent hepatocyte ferroptosis.

ABSTRACT:

Background

Liver ischemia/reperfusion (I/R) injury is usually caused by hepatic inflow occlusion during liver surgery, and is frequently observed during war wounds and trauma. Hepatocyte ferroptosis plays a critical role in liver I/R injury, however, it remains unclear whether this process is controlled or regulated by members of the DEAD/DExH-box helicase (DDX/DHX) family.

Methods

The expression of DDX/DHX family members during liver I/R injury was screened using transcriptome analysis. Hepatocyte-specific Dhx58 knockout mice were constructed, and a partial liver I/R operation was performed. Single-cell RNA sequencing (scRNA-seq) in the liver post I/R suggested enhanced ferroptosis by Dhx58^hep-/-. The mRNAs and proteins associated with DExH-box helicase 58 (DHX58

SUBMITTER: Jia KW 

PROVIDER: S-EPMC11017495 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature