Novel PET Imaging Probe for Quantitative Detection of Senescence In Vivo.

ABSTRACT: Real-time detection of cellular senescence remains a clinical challenge. Here, we aimed to develop a positron emission tomography (PET) imaging probe targeting senescence-associated β-galactosidase (SA-β-Gal), the most widely used biomarker of cellular senescence, and investigate its performance for real-time in vivo quantitative detection of cellular senescence. A stable PET imaging agent [⁶⁸Ga]Ga-BGal was obtained with a high labeling yield (90.0 ± 4.3%) and a radiochemical purity (>95%). [⁶⁸Ga]Ga-BGal displayed high sensitivity and specificity for β-Gal both in vitro and in vivo. The reaction and uptake of the probe correlated with the β-Gal concentration and reaction time. In PET imaging, high β-Gal-expressing CT26.CL25 tu

SUBMITTER: Xiang X 

PROVIDER: S-EPMC11017977 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature