Intra-arterial delivery of neurospheres into isolated perfused porcine colons: a proof of concept.

ABSTRACT: Cell replacement in aganglionic intestines is a promising, yet merely experimental tool for the therapy of congenital dysganglionosis of the enteric nervous system like Hirschsprung disease. While the injection of single cells or neurospheres to a defined and very restricted location is trivial, the translation to the clinical application, where large aganglionic or hypoganglionic areas need to be colonized (hundreds of square centimetres), afford a homogeneous distribution of multiple neurospheres all over the affected tissue areas. Reaching the entire aganglionic area in vivo is critical for the restoration of peristaltic function. The latter mainly depends on an intact nervous system that extends throughout the organ. Intra-arterial injection is a common method in cell therapy an

SUBMITTER: Martel RD 

PROVIDER: S-EPMC11018533 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature