T-cell subsets and cytokines are indicative of neoadjuvant chemoimmunotherapy responses in NSCLC.

ABSTRACT:

Purpose

Neoadjuvant PD-1 blockade combined with chemotherapy is a promising treatment for resectable non-small cell lung cancer (NSCLC), yet the immunological mechanisms contributing to tumor regression and biomarkers corresponding to different pathological responses remain unclear.

Methods

Using dynamic and paired blood samples from NSCLC patients receiving neoadjuvant chemoimmunotherapy, we analyzed the frequencies of CD8 + T-cell and Treg subsets and their dynamic changes during neoadjuvant treatment through flow cytometry. Cytokine profiles and function-related gene expression of CD8 + T cells and Tregs were analyzed through flow cytometry and mRNA-seq. Infiltrating T-cell subsets in resected tissues from patients with different pathological responses were analyzed throu

SUBMITTER: Yi L 

PROVIDER: S-EPMC11018727 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature