An experimental framework to assess biomolecular condensates in bacteria.

ABSTRACT: High-resolution imaging of biomolecular condensates in living cells is essential for correlating their properties to those observed through in vitro assays. However, such experiments are limited in bacteria due to resolution limitations. Here we present an experimental framework that probes the formation, reversibility, and dynamics of condensate-forming proteins in Escherichia coli as a means to determine the nature of biomolecular condensates in bacteria. We demonstrate that condensates form after passing a threshold concentration, maintain a soluble fraction, dissolve upon shifts in temperature and concentration, and exhibit dynamics consistent with internal rearrangement and exchange between condensed and soluble fractions. We also discover that an established marker for insoluble prot

SUBMITTER: Hoang Y 

PROVIDER: S-EPMC11018776 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature