Enterovirus A71 infection-induced dry eye-like symptoms by damaging the lacrimal glands.

ABSTRACT:

Purpose

To determine the effects of EV-A71 (Enterovirus A71) infection on ocular surface and its mechanism.

Methods

AG6 mice aged two to three weeks were randomly divided into control and EV-A71 infected groups. Slit-lamp observation, fluorescein staining, and phenol red thread test were used to assess symptoms of ocular surface at 4 dpi (days post infection). The pathological changes of cornea and lacrimal gland were observed by H&E staining, PAS staining, TUNEL assay, IHC staining and qRT-PCR. Corneas and lacrimal glands from mice were obtained and processed for RNA sequencing analysis. Newly diagnosed HFMD patients caused by EV-A71 were recruited and ensured they met the inclusion criteria. Ocular surface parameters (TMH and NIKBUT) were measured using the OCULUS Keratogr

SUBMITTER: Zhou N 

PROVIDER: S-EPMC11018897 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature