Deep eutectic solvent-based shaking-assisted extraction for determination of bioactive compounds from Norway spruce roots.

ABSTRACT: Polyphenolic compounds play an essential role in plant growth, reproduction, and defense mechanisms against pathogens and environmental stresses. Extracting these compounds is the initial step in assessing phytochemical changes, where the choice of extraction method significantly influences the extracted analytes. However, due to environmental factors, analyzing numerous samples is necessary for statistically significant results, often leading to the use of harmful organic solvents for extraction. Therefore, in this study, a novel DES-based shaking-assisted extraction procedure for the separation of polyphenolic compounds from plant samples followed by LC-ESI-QTOF-MS analysis was developed. The DES was prepared from choline chloride (ChCl) as the hydrogen bond acceptor (HBA) and fructose (

SUBMITTER: Kalyniukova A 

PROVIDER: S-EPMC11018933 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature