High-throughput profiling of metabolic responses to exogenous nutrients in Synechocystis sp. PCC 6803.

ABSTRACT: Cyanobacteria fix carbon dioxide and release carbon-containing compounds into the wider ecosystem, yet they are sensitive to small metabolites that may impact their growth and physiology. Several cyanobacteria can grow mixotrophically, but we currently lack a molecular understanding of how specific nutrients may alter the compounds they release, limiting our knowledge of how environmental factors might impact primary producers and the ecosystems they support. In this study, we develop a high-throughput phytoplankton culturing platform and identify how the model cyanobacterium Synechocystis sp. PCC 6803 responds to nutrient supplementation. We assess growth responses to 32 nutrients at two concentrations, identifying 15 that are utilized mixotrophically. Seven nutrient sources signif

SUBMITTER: Haavisto V 

PROVIDER: S-EPMC11019784 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature