Reduced Monocyte and Neutrophil Infiltration and Activation by P-Selectin/CD62P Inhibition Enhances Thrombus Resolution in Mice.

ABSTRACT:

Background

Venous thromboembolism is a major health problem. After thrombus formation, its resolution is essential to re-establish blood flow, which is crucially mediated by infiltrating neutrophils and monocytes in concert with activated platelets and endothelial cells. Thus, we aimed to modulate leukocyte function during thrombus resolution post-thrombus formation by blocking P-selectin/CD62P-mediated cell interactions.

Methods

Thrombosis was induced by inferior vena cava stenosis through ligation in mice. After 1 day, a P-selectin-blocking antibody or isotype control was administered and thrombus composition and resolution were analyzed.

Results

Localizing neutrophils and macrophages in thrombotic lesions of wild-type mice revealed that these cells enter the thromb

SUBMITTER: Kral-Pointner JB 

PROVIDER: S-EPMC11020038 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature