Two distinct waves of transcriptome and translatome changes drive Drosophila germline stem cell differentiation.

ABSTRACT: The tight control of fate transitions during stem cell differentiation is essential for proper tissue development and maintenance. However, the challenges in studying sparsely distributed adult stem cells in a systematic manner have hindered efforts to identify how the multilayered regulation of gene expression programs orchestrates stem cell differentiation in vivo. Here, we synchronised Drosophila female germline stem cell (GSC) differentiation in vivo to perform in-depth transcriptome and translatome analyses at high temporal resolution. This characterisation revealed widespread and dynamic changes in mRNA level, promoter usage, exon inclusion, and translation efficiency. Transient expression of the master regulator, Bam, drives a first wave of expression changes, primarily modifying th

SUBMITTER: Samuels TJ 

PROVIDER: S-EPMC11021484 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature