Immune system-related plasma extracellular vesicles in healthy aging.

ABSTRACT:

Objectives

To identify age-related plasma extracellular vehicle (EVs) phenotypes in healthy adults.

Methods

EV proteomics by high-resolution mass spectrometry to evaluate EV protein stability and discover age-associated EV proteins (n=4 with 4 serial freeze-thaws each); validation by high-resolution flow cytometry and EV cytokine quantification by multiplex ELISA (n=28 healthy donors, aged 18-83 years); quantification of WI-38 fibroblast cell proliferation response to co-culture with PKH67-labeled young and old plasma EVs. The EV samples from these plasma specimens were previously characterized for bilayer structure, intra-vesicle mitochondria and cytokines, and hematopoietic cell-related surface markers.

Results

Compared with matched exo-EVs (EV-depleted supernatants

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11021711 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature