Immune cells and inflammatory mediators cause endothelial dysfunction in a vascular microphysiological system.

ABSTRACT: Functional assessment of endothelium serves as an important indicator of vascular health and is compromised in vascular disorders including hypertension, atherosclerosis, and preeclampsia. Endothelial dysfunction in these cases is linked to dysregulation of the immune system involving both changes to immune cells and increased secretion of inflammatory cytokines. Herein, we utilize a well-established microfluidic device to generate a 3-dimensional vascular microphysiological system (MPS) consisting of a tubular blood vessel lined with human umbilical vein endothelial cells (HUVECs) to evaluate endothelial function measured via endothelial permeability and Ca²⁺ signaling. We evaluated the effect of a mixture of factors associated with inflammation and cardiovascular diseas

SUBMITTER: Rengarajan A 

PROVIDER: S-EPMC11022267 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature