Computational modeling of light processing in the habenula and dorsal raphe based on laser ablation of functionally-defined cells.

ABSTRACT:

Background

The habenula is a major regulator of serotonergic neurons in the dorsal raphe, and thus of brain state. The functional connectivity between these regions is incompletely characterized. Here, we use the ability of changes in irradiance to trigger reproducible changes in activity in the habenula and dorsal raphe of zebrafish larvae, combined with two-photon laser ablation of specific neurons, to establish causal relationships.

Results

Neurons in the habenula can show an excitatory response to the onset or offset of light, while neurons in the anterior dorsal raphe display an inhibitory response to light, as assessed by calcium imaging. The raphe response changed in a complex way following ablations in the dorsal habenula (dHb) and ventral habenula (vHb). After ablat

SUBMITTER: Cheng RK 

PROVIDER: S-EPMC11022313 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature