Expression patterns of mechanosensitive ion channel PIEZOs in irreversible pulpitis.

ABSTRACT:

Background

Mechanosensitive ion channel PIEZOs have been widely reported to involve inflammation and pain. This study aimed to clarify expression patterns of PIEZOs and their potential relations to irreversible pulpitis.

Materials and methods

Normal pulp tissues (n = 29) from patients with impacted third molars and inflamed pulp tissues (n = 23) from patients with irreversible pulpitis were collected. Pain levels were assessed using a numerical rating scale. PIEZO expressions were measured using real-time PCR and then confirmed using GEO datasets GSE77459, immunoblot, and immunohistochemistry staining. Correlations of PIEZO mRNA expression with inflammatory markers, pain markers, or clinical pain levels were evaluated using Spearman's correlation analysis. Univariate analysi

SUBMITTER: Yang W 

PROVIDER: S-EPMC11022356 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature