Efficient markerless genetic manipulation of Pasteurella multocida using lacZ and pheSm as selection markers.

ABSTRACT: Pasteurella multocida is a zoonotic conditional pathogen that infects multiple livestock species, causing substantial economic losses in the animal husbandry industry. An efficient markerless method for gene manipulation may facilitate the investigations of P. multocida gene function and pathogenesis of P. multocida. Herein, a temperature-sensitive shuttle vector was constructed using lacZ as a selection marker, and markerless glgB, opa, and hyaE mutants of P. multocida were subsequently constructed through blue-white colony screening. The screening efficiency of markerless deletion strains was improved by the lacZ system, and the method could be used for multiple gene deletions. However, the fur mutant was unavailable v

SUBMITTER: Jiang J 

PROVIDER: S-EPMC11022533 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature