Induced Burkholderia prophages detected from the hemoculture: a biomarker for Burkholderia pseudomallei infection.

ABSTRACT: Bacteriophages (phages), viruses that infect bacteria, are found in abundance not only in the environment but also in the human body. The use of phages for the diagnosis of melioidosis, a tropical infectious disease caused by Burkholderia pseudomallei, is emerging as a promising novel approach, but our understanding of conditions under which Burkholderia prophages can be induced remains limited. Here, we first demonstrated the isolation of Burkholderia phages from the hemocultures of melioidosis patients. The B. pseudomallei-positive hemoculture bottles were filtered to remove bacteria, and then phages were isolated and purified by spot and double agar overlay plaque assays. Forty blood samples (hemoculture-confirmed melioidosis) were tested, and phages were fou

SUBMITTER: Withatanung P 

PROVIDER: S-EPMC11022660 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature