Light Color-Controlled pH-Adjustment of Aqueous Solutions Using Engineered Proteoliposomes.

ABSTRACT: Controlling the pH at the microliter scale can be useful for applications in research, medicine, and industry, and therefore represents a valuable application for synthetic biology and microfluidics. The presented vesicular system translates light of different colors into specific pH changes in the surrounding solution. It works with the two light-driven proton pumps bacteriorhodopsin and blue light-absorbing proteorhodopsin Med12, that are oriented in opposite directions in the lipid membrane. A computer-controlled measuring device implements a feedback loop for automatic adjustment and maintenance of a selected pH value. A pH range spanning more than two units can be established, providing fine temporal and pH resolution. As an application example, a pH-sensitive enzyme reaction is prese

SUBMITTER: Harder D 

PROVIDER: S-EPMC11022694 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature