A transient expression tool box for anthocyanin biosynthesis in Nicotiana benthamiana.

ABSTRACT: Transient expression in Nicotiana benthamiana offers a robust platform for the rapid production of complex secondary metabolites. It has proven highly effective in helping identify genes associated with pathways responsible for synthesizing various valuable natural compounds. While this approach has seen considerable success, it has yet to be applied to uncovering genes involved in anthocyanin biosynthetic pathways. This is because only a single anthocyanin, delphinidin 3-O-rutinoside, can be produced in N. benthamiana by activation of anthocyanin biosynthesis using transcription factors. The production of other anthocyanins would necessitate the suppression of certain endogenous flavonoid biosynthesis genes while transiently expressing others. In this work, we present a series of tools fo

SUBMITTER: Grutzner R 

PROVIDER: S-EPMC11022804 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature