Single-cell multiplex chromatin and RNA interactions in ageing human brain.

ABSTRACT: Dynamically organized chromatin complexes often involve multiplex chromatin interactions and sometimes chromatin-associated RNA^1-3. Chromatin complex compositions change during cellular differentiation and ageing, and are expected to be highly heterogeneous among terminally differentiated single cells^4-7. Here we introduce the multinucleic acid interaction mapping in single cells (MUSIC) technique for concurrent profiling of multiplex chromatin interactions, gene expression and RNA-chromatin associations within individual nuclei. When applied to 14 human frontal cortex samples from older donors, MUSIC delineated diverse cortical cell types and states. We observed that nuclei exhibiting fewer short-range chromatin interactions were correlated with both an 'older' trans

SUBMITTER: Wen X 

PROVIDER: S-EPMC11023937 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature