Cross-link assisted spatial proteomics to map sub-organelle proteomes and membrane protein topologies.

ABSTRACT: The functions of cellular organelles and sub-compartments depend on their protein content, which can be characterized by spatial proteomics approaches. However, many spatial proteomics methods are limited in their ability to resolve organellar sub-compartments, profile multiple sub-compartments in parallel, and/or characterize membrane-associated proteomes. Here, we develop a cross-link assisted spatial proteomics (CLASP) strategy that addresses these shortcomings. Using human mitochondria as a model system, we show that CLASP can elucidate spatial proteomes of all mitochondrial sub-compartments and provide topological insight into the mitochondrial membrane proteome. Biochemical and imaging-based follow-up studies confirm that CLASP allows discovering mitochondria-associated proteins and

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC11024108 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature