RNA targeting and cleavage by the type III-Dv CRISPR effector complex.

ABSTRACT: CRISPR-Cas are adaptive immune systems in bacteria and archaea that utilize CRISPR RNA-guided surveillance complexes to target complementary RNA or DNA for destruction^1-5. Target RNA cleavage at regular intervals is characteristic of type III effector complexes^6-8. Here, we determine the structures of the Synechocystis type III-Dv complex, an apparent evolutionary intermediate from multi-protein to single-protein type III effectors^9,10, in pre- and post-cleavage states. The structures show how multi-subunit fusion proteins in the effector are tethered together in an unusual arrangement to assemble into an active and programmable RNA endonuclease and how the effector utilizes a distinct mechanism for target RNA seeding from other type III effectors. Using st

SUBMITTER: Schwartz EA 

PROVIDER: S-EPMC11026444 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature