Transcriptomic profiling across human serotonin neuron differentiation via the FEV reporter system.

ABSTRACT:

Background

The detailed transcriptomic profiles during human serotonin neuron (SN) differentiation remain elusive. The establishment of a reporter system based on SN terminal selector holds promise to produce highly-purified cells with an early serotonergic fate and help elucidate the molecular events during human SN development process.

Methods

A fifth Ewing variant (FEV)-EGFP reporter system was established by CRISPR/Cas9 technology to indicate SN since postmitotic stage. FACS was performed to purify SN from the heterogeneous cell populations. RNA-sequencing analysis was performed for cells at four key stages of differentiation (pluripotent stem cells, serotonergic neural progenitors, purified postmitotic SN and purifed mature SN) to explore the transcriptomic dynamics dur

SUBMITTER: Li Y 

PROVIDER: S-EPMC11027224 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature