Dynamics of peripheral T cell clones during PD-1 blockade in non-small cell lung cancer.

ABSTRACT: Understanding of the functional states and clonal dynamics of T cells after immune checkpoint blockade (ICB) is valuable for improving these therapeutic strategies. Here we performed Smart-seq2 single-cell RNA sequencing (scRNA-seq) analysis on 3,110 peripheral T cells of non-small cell lung cancer (NSCLC) patients before and after the initiation of programmed cell death protein 1 (PD-1) blockade. We identified individual peripheral T cell clones based on the full-length T cell receptor (TCR) sequences and monitored their dynamics during immunotherapy. We found a higher cytotoxic activity in the tumor-related CD4⁺ T cell clones than in the CD8⁺ T cell clones. Based on a large tumor-related CD4⁺ T cell clone, we observed a dramatically decreased abundance af

SUBMITTER: Zhang F 

PROVIDER: S-EPMC11027464 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature