In vitro 4-1BB stimulation promotes expansion of CD8⁺ tumor-infiltrating lymphocytes from various sarcoma subtypes.

ABSTRACT: Tumor-specific tumor-infiltrating lymphocytes (TILs) can be in vitro expanded and have the ability to induce complete and durable tumor regression in some patients with melanoma following adoptive cell therapy (ACT). In this preclinical study, we investigated the feasibility of expanding TIL from sarcomas, as well as performing functional in vitro analyses on these. TILs were expanded in vitro by the use of IL2 stimulation with or without the addition of 4-1BB and CD3 antibodies. Phenotypical and functional analyses were mainly performed by flow cytometry. TILs were expanded from 25 of 28 (89%) tumor samples from patients with 9 different sarcoma subtypes. TILs were predominantly αβ T-cells of effector memory subtype with CD4⁺  dominance. In particular, CD8⁺ TIL highl

SUBMITTER: Nielsen M 

PROVIDER: S-EPMC11027669 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature