Mistranslating the genetic code with leucine in yeast and mammalian cells.

ABSTRACT: Translation fidelity relies on accurate aminoacylation of transfer RNAs (tRNAs) by aminoacyl-tRNA synthetases (AARSs). AARSs specific for alanine (Ala), leucine (Leu), serine, and pyrrolysine do not recognize the anticodon bases. Single nucleotide anticodon variants in their cognate tRNAs can lead to mistranslation. Human genomes include both rare and more common mistranslating tRNA variants. We investigated three rare human tRNA^Leu variants that mis-incorporate Leu at phenylalanine or tryptophan codons. Expression of each tRNA^Leu anticodon variant in neuroblastoma cells caused defects in fluorescent protein production without significantly increased cytotoxicity under normal conditions or in the context of proteasome inhibition. Using tRNA sequencing and mass spectro

SUBMITTER: Davey-Young J 

PROVIDER: S-EPMC11028032 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature