Comparison of IL-2 vs IL-7/IL-15 for the generation of NY-ESO-1-specific T cells.

ABSTRACT: The anti-tumor efficacy of TCR-engineered T cells in vivo depends largely on less-differentiated subsets such as T cells with naïve-like T cell (T_N) phenotypes with greater expansion and long-term persistence. To increase these subsets, we compared the generation of New York esophageal squamous cell carcinoma-1 (NY-ESO-1)-specific T cells under supplementation with either IL-2 or IL-7/IL-15. PBMCs were transduced with MS3II-NY-ESO-1-siTCR retroviral vector. T cell generation was adapted from a CD19-specific CART cell production protocol. Comparable results in viability, expansion and transduction efficiency of T cells under stimulation with either IL-2 or IL-7/IL-15 were observed. IL-7/IL-15 led to an increase of CD4⁺ T cells and a decrease of CD8⁺ T cells,

SUBMITTER: Gong W 

PROVIDER: S-EPMC11028180 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature