A multiplex method for the detection of serum antibodies against in silico-predicted tumor antigens.

ABSTRACT: Humoral immune responses against tumor antigens are studied as indirect markers of antigen exposure and in cancer vaccine studies. An increasing number of tumor antigens potentially translated from mutant genes is identified by advances in genomic sequencing. They represent an interesting source for yet unknown immunogenic epitopes. We here describe a multiplex method using the Luminex technology allowing for the detection of antibodies against multiple in silico-predicted linear neo-antigens in large sets of sera. The approach included 32 synthetic biotinylated peptides comprising a predicted set of frameshift mutation-induced neo-antigens. The antigens were fused to a FLAG epitope to ensure monitoring antigen binding to avidin-linked microspheres in the absence of monoclonal antibodies.

SUBMITTER: Reuschenbach M 

PROVIDER: S-EPMC11029127 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature