Classification and functional characterization of regulators of intracellular STING trafficking identified by genome-wide optical pooled screening.

ABSTRACT: STING is an innate immune sensor that traffics across many cellular compartments to carry out its function of detecting cyclic di-nucleotides and triggering defense processes. Mutations in factors that regulate this process are often linked to STING-dependent human inflammatory disorders. To systematically identify factors involved in STING trafficking, we performed a genome-wide optical pooled screen and examined the impact of genetic perturbations on intracellular STING localization. Based on subcellular imaging of STING protein and trafficking markers in 45 million cells perturbed with sgRNAs, we defined 464 clusters of gene perturbations with similar cellular phenotypes. A higher-dimensional focused optical pooled screen on 262 perturbed genes which assayed 11 imaging channels identifi

SUBMITTER: Gentili M 

PROVIDER: S-EPMC11030420 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature