Self-incompatibility phenotypes of SRK mutants can be predicted with high accuracy.

ABSTRACT: Only very limited information is available on why some non-synonymous variants severely alter gene function while others have no effect. To identify the characteristic features of mutations that strongly influence gene function, this study focused on S-locus receptor kinase, SRK, which encodes a highly polymorphic receptor kinase expressed in stigma papillary cells that underlies a female determinant of self-incompatibility in Brassicaceae. A set of 299 Arabidopsis thaliana transformants expressing mutated SRKb from A. lyrata was constructed and analyzed to determine the genotype and self-incompatibility phenotype of each transformant. Almost all the transformants showing the self-incompatibility defect contained mutations in AlSRKb that altered localization to

SUBMITTER: Yamamoto M 

PROVIDER: S-EPMC11030437 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature