Glycosylation as a tracer of off-target Cre-lox activation in development.

ABSTRACT: The Cre-lox system is one of the most widely used methods for lineage-specific and inducible genome editing in vivo. However, incomplete penetrance and off-target effects due to transient promoter expression in a stem or pluripotent precursor cell can be problematic and difficult to detect, especially if the target gene is not normally present in the fully differentiated but off-target cells. Yet, the loss of the target gene through the transient expression of Cre may impact the differentiation of those cells by virtue of transient expression in a precursor population. In these situations, off-target effects in an unknown precursor cell can, at best, complicate conclusions drawn from the model, and at worst, invalidate all data generated from that knockout strain. Thus, identifying Cre-dri

SUBMITTER: Glendenning LM 

PROVIDER: S-EPMC11031139 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature