Gn protein expressed in plants for diagnosis of severe fever with thrombocytopenia syndrome virus.

ABSTRACT: Severe fever with thrombocytopenia syndrome virus (SFTSV) causes the highly fatal disease in humans. To facilitate diagnosis, the native form of subunit glycoprotein (Gn), a prime target for potential vaccines and therapies, was produced in Nicotiana benthamiana using a Bamboo mosaic virus-based vector system. By fusion with secretory signal tags, SS^Ext, derived from the extension protein, and the (SP)₁₀ motif, the yield of the recombinant Gn (rGn) was remarkably increased to approximately 7 mg/kg infiltrated leaves. Ultimately, an rGn-based ELISA was successfully established for the detection of SFTSV-specific antibodies in serum samples from naturally infected monkeys. As validated with the reference method, the specificity and sensitivity of rGn-ELISA were 94% and

SUBMITTER: Chang YC 

PROVIDER: S-EPMC11031438 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature