Novel ultrasensitive immunoassay for the selective quantification of tau oligomers and related soluble aggregates.

ABSTRACT:

Introduction

Tau aggregation into paired helical filaments and neurofibrillary tangles is characteristic of Alzheimer's disease (AD) and related disorders. However, biochemical assays for the quantification of soluble, earlier-stage tau aggregates are lacking. We describe an immunoassay that is selective for tau oligomers and related soluble aggregates over monomers.

Methods

A homogeneous (single-antibody) immunoassay was developed using a novel anti-tau monoclonal antibody and validated with recombinant and brain tissue-derived tau.

Results

The assay signals were concentration dependent for recombinant tau aggregates in solution but not monomers, and recognized peptides within, but not outside, the aggregation-prone microtubule binding region. The signals in inferior

SUBMITTER: Islam T 

PROVIDER: S-EPMC11032517 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature