Discovery and validation of molecular patterns and immune characteristics in the peripheral blood of ischemic stroke patients.

ABSTRACT:

Background

Stroke is a disease with high morbidity, disability, and mortality. Immune factors play a crucial role in the occurrence of ischemic stroke (IS), but their exact mechanism is not clear. This study aims to identify possible immunological mechanisms by recognizing immune-related biomarkers and evaluating the infiltration pattern of immune cells.

Methods

We downloaded datasets of IS patients from GEO, applied R language to discover differentially expressed genes, and elucidated their biological functions using GO, KEGG analysis, and GSEA analysis. The hub genes were then obtained using two machine learning algorithms (least absolute shrinkage and selection operator (LASSO) and support vector machine-recursive feature elimination (SVM-RFE)) and the immune cell infiltr

SUBMITTER: Cong L 

PROVIDER: S-EPMC11034498 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature