Development of an attenuated potato virus Y mutant carrying multiple mutations in helper-component protease for cross-protection.

ABSTRACT: Tobacco (Nicotiana tabacum) is one of the major cash crops in China. Potato virus Y (PVY), a representative member of the genus Potyvirus, greatly reduces the quality and yield of tobacco leaves by inducing veinal necrosis. Mild strain-mediated cross-protection is an attractive method of controlling diseases caused by PVY. Currently, there is a lack of effective and stable attenuated PVY mutants. Potyviral helper component-protease (HC-Pro) is a likely target for the development of mild strains. Our previous studies showed that the residues lysine at positions 124 and 182 (K¹²⁴ and K¹⁸²) in HC-Pro were involved in PVY virulence, and the conserved KITC motif in HC-Pro was involved in aphid transmission. In this study, to improve the stability of PVY mild strains, K at

SUBMITTER: Xu XJ 

PROVIDER: S-EPMC11035042 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature