Dynamics-driven allosteric stimulation of diguanylate cyclase activity in a red light-regulated phytochrome.

ABSTRACT: Sensor-effector proteins integrate information from different stimuli and transform this into cellular responses. Some sensory domains, like red-light responsive bacteriophytochromes, show remarkable modularity regulating a variety of effectors. One effector domain is the GGDEF diguanylate cyclase catalyzing the formation of the bacterial second messenger cyclic-dimeric-guanosine monophosphate. While critical signal integration elements have been described for different phytochromes, a generalized understanding of signal processing and communication over large distances, roughly 100 Å in phytochrome diguanylate cyclases, is missing. Here we show that dynamics-driven allostery is key to understanding signal integration on a molecular level. We generated protein variants stabilized in their

SUBMITTER: Tran QH 

PROVIDER: S-EPMC11035067 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature