Pooled effector library screening in protoplasts rapidly identifies novel Avr genes.

ABSTRACT: Crop breeding for durable disease resistance is challenging due to the rapid evolution of pathogen virulence. While progress in resistance (R) gene cloning and stacking has accelerated in recent years^1-3, the identification of corresponding avirulence (Avr) genes in many pathogens is hampered by the lack of high-throughput screening options. To address this technology gap, we developed a platform for pooled library screening in plant protoplasts to allow rapid identification of interacting R-Avr pairs. We validated this platform by isolating known and novel Avr genes from wheat stem rust (Puccinia graminis f. sp. tritici) after screening a designed library of putative effectors against individual R genes. Rapid Avr gene identification provides molecular tools to understand and t

SUBMITTER: Arndell T 

PROVIDER: S-EPMC11035141 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature