Caged Zn²⁺ Photolysis in Zebrafish Whole Brains Reveals Subsecond Modulation of Dopamine Uptake.

ABSTRACT: Free, ionic zinc (Zn²⁺) modulates neurotransmitter dynamics in the brain. However, the sub-s effects of transient concentration changes of Zn²⁺ on neurotransmitter release and uptake are not well understood. To address this lack of knowledge, we have combined the photolysis of the novel caged Zn²⁺ compound [Zn(DPAdeCageOMe)]⁺ with fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes in live, whole brain preparations from zebrafish (Danio rerio). After treating the brain with [Zn(DPAdeCageOMe)]⁺, Zn²⁺ was released by application of light that was gated through a computer-controlled shutter synchronized with the FSCV measurements and delivered through a 1 mm fiber optic cable. We systematically optimized

SUBMITTER: Hettiarachchi P 

PROVIDER: S-EPMC11036533 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature