Activin A signaling stimulates neutrophil activation and macrophage migration in pancreatitis.

ABSTRACT: Acute Pancreatitis (AP) is associated with high mortality and current treatment options are limited to supportive care. We found that blockade of activin A (activin) in mice improves outcomes in two murine models of AP. To test the hypothesis that activin is produced early in response to pancreatitis and is maintained throughout disease progression to stimulate immune cells, we first performed digital spatial profiling (DSP) of human chronic pancreatitis (CP) patient tissue. Then, transwell migration assays using RAW264.7 mouse macrophages and qPCR analysis of "neutrophil-like" HL-60 cells were used for functional correlation. Immunofluorescence and western blots on cerulein-induced pancreatitis samples from pancreatic acinar cell-specific Kras knock-in (Ptf1aCre^ER™; LSL-Kras

SUBMITTER: Wiley MB 

PROVIDER: S-EPMC11039671 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature