An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers.

ABSTRACT: Bretschneidera sinensis is a monotypic species of rare and tertiary relic trees mainly distributed in China. B. sinensis is a potentially valuable horticultural plant, which has significant ornamental and research value, and is a crucial tool for the study of phylogeography. The artificial cultivation of B. sinensis is of great scientific value and practical significance. In this study, we developed a direct organogenesis process of B. sinensis using mature zygotic embryos as initial materials. The highest sterile germination induction (54.5%) from the mature zygotic embryo was obtained in a Murashige and Skoog (MS) medium with 2.0 mg·L^-1 6-benzylaminopurine (6-BA) and 0.2 mg·L^-1 α-naphthaleneacetic acid (NAA). The highest percentage of sho

SUBMITTER: Yan X 

PROVIDER: S-EPMC11039884 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature