The repertoire and structure of adhesion GPCR transcript variants assembled from publicly available deep-sequenced human samples.

ABSTRACT: Alternative splicing and multiple transcription start and termination sites can produce a diverse repertoire of mRNA transcript variants from a given gene. While the full picture of the human transcriptome is still incomplete, publicly available RNA datasets have enabled the assembly of transcripts. Using publicly available deep sequencing data from 927 human samples across 48 tissues, we quantified known and new transcript variants, provide an interactive, browser-based application Splice-O-Mat and demonstrate its relevance using adhesion G protein-coupled receptors (aGPCRs) as an example. On average, 24 different transcript variants were detected for each of the 33 human aGPCR genes, and several dominant transcript variants were not yet annotated. Variable transcription starts and comple

SUBMITTER: Kuhn CK 

PROVIDER: S-EPMC11039983 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature