Detection of clinical Serratia marcescens isolates carrying bla_KPC-2 in a hospital in China.

ABSTRACT: Serratia marcescens is an opportunistic and nosocomial pathogen found in the intensive care unit (ICU), but its antimicrobial resistance (AMR) is rarely addressed. Here, we reported two bla_KPC-2-positive S. marcescens strains, SMBC31 and SMBC50, recovered from the ICU of a hospital in Zhengzhou, China. The minimum inhibitory concentration (MIC) was determined using the broth microdilution method, while S1-PFGE was employed to demonstrate plasmid size approximation. Complete genome sequences were obtained through Illumina NovaSeq 6000 and Oxford Nanopore Technologies. Both strains exhibit resistance to meropenem and harbor the bla_KPC-2 and bla_SRT-1 resistance genes. The plasmid pSMBC31-39K in strain SMBC31 and pSMBC50-107K

SUBMITTER: Tang B 

PROVIDER: S-EPMC11040119 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature